Roberto Fiammengo

Researcher
Team Leader

About

Education

1998-2002            Ph. D. in Chemistry University of Twente, The Netherlands.

1991-1997              Degree in Chemistry (grade 110/110) University of Padova, Italy.

Professional Experience

03/12-now             Team Leader - Researcher, Center for Biomolecular Nanotechnologies, Italian Institute of Technology, Lecce, Italy.

Peptide and oligonucleotide modified gold nanoparticles, multifunctional nanoparticles.

11/2006-02/2012   Group leader, Department of New Materials and Biosystems, Max-Planck-Institute for Metals Research, Stuttgart, Germany.

Preparation of peptide-modified gold nanoparticles for the site-selective targeting of NMDA-receptors. PEG-based hydrogels as artificial extracellular matrix.

03/2003-11/2006   Post-doctoral researcher, Institute of Pharmacy and Molecular Biotechnology (IPMB), University of Heidelberg, Germany.

Synthesis of modified nucleosides and nucleic acids. Hybrid catalysts and study of organometallic transformations in aqueous medium.

07/2002-03/2003   Post-doctoral researcher, Organic Chemistry Department, University of Padova, Italy.

Synthesis and study of synthetic metallonucleases.

09/1998-06/2002   Ph. D. student, Laboratory of Supramolecular Chemistry and Technology and MESA+ Research Institute, University of Twente, The Netherlands (Prof. David N. Reinhoudt).

Supramolecular chemistry, self-assembly in aqueous solutions, synthesis of biomimetic O2 binders and activators.

11/1997-08/1998     Research fellow, Organic Chemistry department, University of Padova.

Peptide chemistry and synthesis of novel conformationally constrained peptides as ligands for enantioselective metal-catalyzed reactions.

06/1996-10/1997     Master thesis student, degree in Chemistry + four months research fellow at the Organic Chemistry department, University of Padova (Prof. Fulvio di Furia, Dr. Giulia Licini).

Ti-catalyzed sulfoxidation reactions and cycloaddition reactions.

 

Languages           Italian (mother tongue), English (fluent), German (fluent)

Projects

Current research projects:

Development of functionalizable poly(ethylene glycol) (PEG)-based coatings for nanoparticles

Application of biofunctionalized gold nanoparticles:

  1. in diagnostics: quantification of microRNAs in sample of extracted total RNA.
  2. in nanomedicine: selective targeting of receptors on the cell surface (NMDA receptor, leptin receptor); development of nanoparticle-based anticancer vaccines.

Development of silver-nanoparticle-based conductive inks.


External funding:


2014 - 2015 ISSF Network of Excellence scheme award (Wellcome Trust Institutional Strategic Support Fund) with Dr Iain Dunlop, Professor David Dexter, Dr Alexandra Porter (Departments of Materials and Medicine, Imperial College London, UK).

2017 - 2020 CoPI PRIN project 2015RNWJAM " Nanoplatforms for enhanced immune responses " financed by the Italian Ministry of Education, Universities and Research (total 329 k€)

Selected Publications

  1. Scarpa, E.; Lemma, E. D.; Fiammengo, R.; Cipolla, M. P.; Pisanello, F.; Rizzi, F.; De Vittorio, M. Microfabrication of pH-responsive 3D hydrogel structures via two-photon polymerization of high-molecular-weight poly(ethylene glycol) diacrylates. Sens. Actuators, B 2018, doi: 10.1016/j.snb.2018.09.079.

  2. Maggi, V.; Bianchini, F.; Portioli, E.; Peppicelli, S.; Lulli, M.; Bani, D.; Del Sole, R.; Zanardi, F.; Sartori, A.; Fiammengo, R.* Gold nanoparticles functionalized with RGD-semipeptides: a simple yet highly effective targeting system for αVβ3 integrins. Chem. Eur. J. 2018, 24, 12093.

  3. Fiammengo, R.* Can nanotechnology improve cancer diagnosis through miRNA detection? Biomarkers Med. 2017, 11, 69-86.

  4. Perinot, A.; Kshirsagar, P.; Malvindi, M. A.; Pompa, P. P.; Fiammengo, R.*; Caironi, M.* Direct-written polymer field-effect transistors operating at 20 MHz. Sci. Rep. 2016, 6, 38941.

  5. Gonzalez-Carter, D.; Goode, A. E.; Fiammengo, R.; Dunlop, I. E.; Dexter, D. T.; Porter, A. E. Inhibition of leptin–ObR interaction does not prevent leptin translocation across a human blood‑brain barrier model. J. Neuroendocrinol. 2016, doi: 10.1111/jne.12392.

  6. Cai, H.; Degliangeli, F.; Palitzsch, B.; Gerlitzki, B.; Kunz, H.; Schmitt, E.*; Fiammengo, R.*; Westerlind, U.* Glycopeptide-functionalized gold nanoparticles for antibody induction against the tumor associated mucin-1 glycoprotein. Bioorg. Med. Chem. 2016, 24, 1132–1135.

  7. Degliangeli, F.; Pompa, P. P.; Fiammengo, R.* Nanotechnology-based strategies for the detection and quantification of microRNA. Chem. Eur. J. 2014, 20, 9476–9492.

  8. Degliangeli, F.; Kshirsagar, P.; Brunetti, V.; Pompa, P. P.*; Fiammengo, R.* Absolute and direct microRNA quantification using DNA–gold nanoparticle probes. J. Am. Chem. Soc. 2014, 136, 2264-2267.

  9. Valentini, P.; Fiammengo, R.; Sabella, S.; Gariboldi, M.; Maiorano, G.; Cingolani, R.; Pompa, P. P. Gold-nanoparticle-based colorimetric discrimination of cancer-related point mutations with picomolar sensitivity. ACS Nano 2013, 7, 5530-5538.

  10. Brunetti, V.; Chibli, H.; Fiammengo, R.; Galeone, A.; Malvindi, M.A.; Vecchio, G.; Cingolani, R.; Nadeau, J.L.; Pompa, P.P. InP/ZnS as a safer alternative to CdSe/ZnS core/shell quantum dots: in vitro and in vivo toxicity assessment. Nanoscale 2013, 5, 307-317.

  11. Lee-Thedieck, C.; Rauch, N.; Fiammengo, R.; Klein, G.; Spatz, J.P. Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility. J. Cell Sci. 2012, 125, 3765-3775.

  12. Maus, L.; Dick, O.; Bading, H.; Spatz, J. P.; Fiammengo, R.* Conjugation of Peptides to the Passivation Shell of Gold Nanoparticles for Targeting of Cell-Surface Receptors. ACS Nano 2010, 4, 6617-6628.

  13. Aydin, D.; Louban, I.; Perschmann, N.; Blummel, J.; Lohmueller, T.; Cavalcanti-Adam, E. A.; Haas, T. L.; Walczak, H.; Kessler, H.; Fiammengo, R.*, Spatz, J. P.* Polymeric substrates with tunable elasticity and nanoscopically controlled biomolecule presentation. Langmuir 2010, 26, 15472-15480.

  14. Maus, L.; Spatz, J. P.; Fiammengo, R.* Quantification and reactivity of functional groups in the ligand shell of PEGylated gold nanoparticles via a fluorescence-based assay. Langmuir 2009, 25, 7910-7917.

IIT Publications

  • 2018
  • Salbini M.iit, Stomeo T.iit, Ciracì C., Fiammengo R.iit, Verri T., Smith D.R., De Vittorio M.iit

    Biomechanical nanosensors based on plasmonic nanostructures

    Workshop Annuale Lebiotech
  • Maggi V.iit, Bianchini F., Portioli E., Peppicelli S., Lulli M., Bani D., Del Sole R., Zanardi F., Sartori A., Fiammengo R.iit

    Cyclic aminoproline-RGD functionalized gold nanoparticles for effective targeting of αVβ3 integrins and selective tumor cell internalization

    7th EuCheMS Chemistry Congress
  • Maggi V.iit, Bianchini F., Portioli E., Peppicelli S., Lulli M., Bani D., Sole R.D., Zanardi F., Sartori A., Fiammengo R.iit
    DOI

    Gold Nanoparticles Functionalized with RGD-Semipeptides: A Simple yet Highly Effective Targeting System for αVβ3 Integrins

    Chemistry - A European Journal, vol. 24, (no. 46), pp. 12093-12100
  • Scarpa E.iit, Lemma E.D., Fiammengo R.iit, Cipolla M.P., Pisanello F.iit, Rizzi F.iit, De Vittorio M.iit

    Microfabrication of pH-responsive 3D hydrogel structures via two-photon polymerization of high-molecular-weight poly(ethylene glycol) diacrylates

    Sensors and Actuators, B: Chemical
  • Mangini V.iit, Guerreiro A., Compañón I., Tavano R., Bernardes G. J. L., Corzana F., Papini E., Fiammengo R.iit

    Nanovaccines for the treatment of cancer: multivalent presentation of TA-MUC1 glycopeptides on gold nanoparticles

    E-MRS Spring Meeting 2018
  • Mangini V.iit, Guerreiro A., Compañón I., Tavano R., Bernardes G. J. L., Corzana F., Papini E., Fiammengo R.iit

    Nanovaccines for the treatment of cancer: multivalent presentation of TA-MUC1 glycopeptides on gold nanoparticles

    E-MRS Spring Meeting 2018
  • Mangini V.iit, Guerreiro A., Companon I., Tavano R., Bernardes G. J. L., Corzana F., Papini E., Fiammengo R.iit

    Next generation gold-nanoparticle formulations for improving efficacy of TA-MUC1-directed cancer vaccines

    Medical Biotechnology VIB Conference Series
  • Fiammengo R.iit, Maggi V.iit, Bianchini F., Portioli E., Peppicelli S., Lulli M., Bani D., Del Sole R., Zanardi F., Sartori A.

    Selective targeting of αVβ3 integrins with gold nanoparticles carrying RGD-semipeptides

    XXXVIII Convegno Nazionale della Divisione di Chimica Organica della SCI - CDCO 2018
  • Maggi V.iit, Mangini V.iit, Trianni A., Melle F., Fiammengo R.iit

    Site-Selective and Controlled Immobilization of Leptin on Nanoparticles for improving cellular uptake

    256th ACS National Meeting & Exposition
  • Scarpa E.iit, Lemma E.D., Rizzi F.iit, Fiammengo R.iit, Cipolla M.P., Pisanello F.iit, De Vittorio M.iit

    Two-photon polymerization for fabrication of pH responsive high molecular weight PEG-DA based hydrogel microstructures

    28th Anniversary World Congress on Biosensors
  • 2017
  • Carpegna R.iit, Maggi V.iit, Fiammengo R.iit

    Advanced bioconjugation strategies for engineering the gold nanoparticles surface

    MYCS - Merck Young Chemists Symposium
  • Maggi V.iit, Bianchini F., Sartori A., Fiammengo R.iit

    Aminoproline-RGD functionalized gold nanoparticles for targeting of integrins involved in tumor angiogenesis

    XXVI Congresso Nazionale della Società Chimica Italiana
  • Fiammengo R.iit
    DOI

    Can nanotechnology improve cancer diagnosis through miRNA detection?

    Biomarkers in Medicine, vol. 11, (no. 1), pp. 69-86
  • Maggi V.iit, Bianchini F., Sartori A., Fiammengo R.iit

    Cyclic-aminoproline-RGD gold nanoparticles for targeting integrin tumor angiogenesis

    School of Nanomedicine
  • Fiammengo R.iit, Cai H., Degliangeli F., Liu J., Pett C., Hu J., Kunz H., Westerlind U., Lu M.

    Eliciting specific humoral and cellular immune response by self-adjuvanting gold nanoparticles carrying tumor-associated MUC1 glycopeptides

    XXVI Congresso Nazionale della Società Chimica Italiana
  • Fiammengo R.iit, Maggi V.iit, Mangini V.iit, Carpegna R.iit

    Gold nanoparticles for biological applications and nanomedicine

    School of Nanomedicine
  • Mangini V.iit, Fiammengo R.iit

    Improved size-tunable synthesis of gold nanorods and surface functionalization strategies for biomedical applications

    XXVI Congresso Nazionale della Società Chimica Italiana
  • Mangini V.iit, Fiammengo R.iit

    Improved size-tunable synthesis of gold nanorods and surface functionalization strategies for biomedical applications

    School of Nanomedicine
  • 2016
  • Maggi V.iit, Bianchini F., Del Sole R., Portioli E., Sartori A., Fiammengo R.iit

    Cyclic RGD functionalized PEGylated gold nanoparticles for tumor targeting

    NANOMEDICINE VITERBO 2016
  • Perinot A.iit, Kshirsagar P.iit, Malvindi M.A.iit, Pompa P.P.iit, Fiammengo R.iit, Caironi M.iit
    DOI

    Direct-written polymer field-effect transistors operating at 20 MHz

    Scientific Reports, vol. 6
  • Cai H., Degliangeli F.iit, Palitzsch B., Gerlitzki B., Kunz H., Schmitt E., Fiammengo R.iit, Westerlind U.
    DOI

    Glycopeptide-functionalized gold nanoparticles for antibody induction against the tumor associated mucin-1 glycoprotein

    Bioorganic and Medicinal Chemistry, vol. 24, (no. 5), pp. 1132-1135
  • Maggi V.iit, Bianchini F., Del Sole R., Portioli E., Sartori A., Fiammengo R.iit

    Gold Nanoparticles functionalized with cyclic γ-aminoproline RGD peptide for selective αvβ3 integrin targeting

    MYCS - Merck Young Chemists Symposium
  • Gonzalez-Carter D., Goode A.E., Fiammengo R.iit, Dunlop I.E., Dexter D.T., Porter A.E.
    DOI

    Inhibition of Leptin–ObR Interaction Does not Prevent Leptin Translocation Across a Human Blood–Brain Barrier Model

    Journal of Neuroendocrinology, vol. 28, (no. 6)
  • 2015
  • Fiammengo R.iit, Cai H., Degliangeli F., Palitzsch B., Gerlitzki B., Schmitt E., Westerlind U.

    Glycopeptide-functionalized gold nanoparticles for antibody induction against the tumor associated Mucin-1 glycoprotein

    XXXVI Convegno della Divisione di Chimica Organica – CDCO 2015
  • Fiammengo R.iit, Cai H., Degliangeli F., Palitzsch B., Gerlitzki B., Schmitt E., Westerlind U.

    Towards a Gold Nanoparticle-based Vaccine Directed against the Tumor Associated Mucin-1 Glycoprotein

    TNT2015 - Trends in Nanotechnology
  • 2014
  • Degliangeli F.iit, Kshirsagar P.iit, Brunetti V.iit, Pompa P.P.iit, Fiammengo R.iit
    DOI

    Absolute and direct microRNA quantification using DNA-gold nanoparticle probes

    Journal of the American Chemical Society, vol. 136, (no. 6), pp. 2264-2267
  • Degliangeli F.iit, Pompa P.P.iit, Fiammengo R.iit
    DOI

    Nanotechnology-based strategies for the detection and quantification of microRNA

    Chemistry - A European Journal, vol. 20, (no. 31), pp. 9476-9492
  • Fiammengo R.iit, Degliangeli F., Kshirsagar P., Brunetti V., Pompa P. P.iit

    Sensitive MicroRNA Quantification Using DNA-Gold Nanoparticle Probes

    Nanomedicine Viterbo 2014
  • 2013
  • Fiammengo R.iit, Degliangeli F., Pompa P. P.iit

    DNA-AuNP probes for the sensitive quantification of miRNA

    E-MRS Spring Meeting 2013
  • Valentini P.iit, Fiammengo R.iit, Sabella S.iit, Gariboldi M., Maiorano G.iit, Cingolani R.iit, Pompa P.P.iit
    DOI

    Gold-nanoparticle-based colorimetric discrimination of cancer-related point mutations with picomolar sensitivity

    ACS Nano, vol. 7, (no. 6), pp. 5530-5538
  • Brunetti V.iit, Chibli H., Fiammengo R.iit, Galeone A.iit, Malvindi M.A.iit, Vecchio G.iit, Cingolani R.iit, Nadeau J.L., Pompa P.P.iit
    DOI

    InP/ZnS as a safer alternative to CdSe/ZnS core/shell quantum dots: In vitro and in vivo toxicity assessment

    Nanoscale, vol. 5, (no. 1), pp. 307-317
  • Degliangeli F., Fiammengo R.iit, Pompa P. P.iit

    Quantification of oligonucleotides via efficient processing of DNA-gold nanoparticles by a nuclease

    E-MRS Spring Meeting 2013
  • 2012
  • Lee-Thedieck C., Rauch N., Fiammengo R., Klein G., Spatz J.P.
    DOI

    Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

    Journal of Cell Science, vol. 125, (no. 16), pp. 3765-3775
  • 2011
  • Fiammengo R., Maus L., Lint A., Spatz J. P.

    Conjugation of biologically active molecules to the passivation layer of gold nanoparticles for cellular targeting

    E-MRS Spring Meeting 2011
  • Fiammengo R., Maus L., Lint A., Spatz J. P.

    Conjugation of biologically active peptides to the passivation layer of gold nanoparticles for cellular targeting

    9th Stadler Minerva student workshop – Soft matter: where physics, chemistry, and biology meet, Beer Sheva & Ein-Gedi, Israel
  • 2010
  • Maus L., Dick O., Bading H., Spatz J.P., Fiammengo R.
    DOI

    Conjugation of peptides to the passivation shell of gold nanoparticles for targeting of cell-surface receptors

    ACS Nano, vol. 4, (no. 11), pp. 6617-6628
  • Fiammengo R., Maus L., Spatz J. P.

    Gold nanoparticles for the study of N-methyl-D-aspartate receptors

    ACS 239th National Meeting
  • Fiammengo R., Lint A., Spatz J. P.

    Gold nanoparticles with orthogonally functionalizable groups

    19th Nachwuchswissenschaftler-Symposium bioorganische Chemie, University of Göttingen, Germany
  • Aydin D., Louban I., Perschmann N., Blummel J., Lohmuller T., Cavalcanti-Adam E.A., Haas T.L., Walczak H., Kessler H., Fiammengo R., Spatz J.P.
    DOI

    Polymeric substrates with tunable elasticity and nanoscopically controlled biomolecule presentation

    Langmuir, vol. 26, (no. 19), pp. 15472-15480
  • 2009
  • Fournier P., Fiammengo R., Jaschke A.
    DOI

    Allylic amination by a DNA-diene-iridium(I) hybrid catalyst

    Angewandte Chemie - International Edition, vol. 48, (no. 24), pp. 4426-4429
  • Fiammengo R., Maus L., Spatz J. P.

    Conantokin G functionalized gold nanoparticles for the study of N-methyl-D-aspartate receptors

    18th Nachwuchswissenschaftler-Symposium bioorganische Chemie, University of Hannover, Germany
  • Maus L., Spatz J.P., Fiammengo R.
    DOI

    Quantification and reactivity of functional groups in the Ligand Shell of PEGylated gold nanoparticles via a fluorescence-based assay

    Langmuir, vol. 25, (no. 14), pp. 7910-7917
  • 2008
  • Fiammengo R., Maus L., Spatz J. P.

    Characterization and stability of peptide functionalized gold nanoparticles

    International symposium “Colloidal nanoparticles – From synthesis to biological applications”, Marburg, Germany
  • Fiammengo R., Maus L., Spatz J. P.

    Counting the number of peptides coupled to the surface of mixed-monolayer protected gold nanoparticles

    EMBL Conference on Chemical Biology
  • 2007
  • Caprioara M., Fiammengo R., Engeser M., Jaschke A.
    DOI

    DNA-based phosphane ligands

    Chemistry - A European Journal, vol. 13, (no. 7), pp. 2089-2095
  • Martin M., Manea F., Fiammengo R., Prins L.J., Pasquato L., Scrimin P.
    DOI

    Metallodendrimers as transphosphorylation catalysts

    Journal of the American Chemical Society, vol. 129, (no. 22), pp. 6982-6983
  • Pfander S., Fiammengo R., Kirin S.I., Metzler-Nolte N., Jaschke A.
    DOI

    Reversible site-specific tagging of enzymatically synthesized RNAs using aldehyde-hydrazine chemistry and protease-cleavable linkers

    Nucleic Acids Research, vol. 35, (no. 4)
  • 2005
  • Helm M., Petermeier M., Ge B., Fiammengo R., Jaschke A.
    DOI

    Allosterically activated Diels-Alder catalysis by a ribozyme

    Journal of the American Chemical Society, vol. 127, (no. 30), pp. 10492-10493
  • Fiammengo R., Musilek K., Jaschke A.
    DOI

    Efficient preparation of organic substrate-RNA conjugates via in vitro transcription

    Journal of the American Chemical Society, vol. 127, (no. 25), pp. 9271-9276
  • Fiammengo R., Jaschke A.
    DOI

    Nucleic acid enzymes

    Current Opinion in Biotechnology, vol. 16, (no. 6), pp. 614-621
  • 2003
  • Fiammengo R., Wojciechowski K., Crego-Calama M., Timmerman P., Figoli A., Wessling M., Reinhoudt D.N.
    DOI

    Heme-Protein Active Site Models via Self-Assembly in Water

    Organic Letters, vol. 5, (no. 19), pp. 3367-3370
  • Fiammengo R., Crego-Calama M., Timmerman P., Reinhoudt D.N.
    DOI

    Recognition of caffeine in aqueous solutions

    Chemistry - A European Journal, vol. 9, (no. 3), pp. 784-792
  • 2002
  • Corbellini F., Fiammengo R., Timmerman P., Crego-Calama M., Versluis K., Heck A.J.R., Luyten I., Reinhoudt D.N.
    DOI

    Guest encapsulation and self-assembly of molecular capsules in polar solvents via multiple ionic interactions

    Journal of the American Chemical Society, vol. 124, (no. 23), pp. 6569-6575
  • Fiammengo R., Bruinink C.M., Crego-Calama M., Reinhoudt D.N.
    DOI

    Noncovalent secondary interactions in Co(II)salen complexes: O2 binding and catalytic activity in cyclohexene oxygenation

    Journal of Organic Chemistry, vol. 67, (no. 24), pp. 8552-8557
  • Fiammengo R., Timmerman P., Huskens J., Versluis K., Heck A.J.R., Reinhoudt D.N.
    DOI

    Non-covalent synthesis of calix[4]arene-capped porphyrins in polar solvents via ionic interactions

    Tetrahedron, vol. 58, (no. 4), pp. 757-764
  • 2001
  • Michels J.J., Fiammengo R., Timmerman P., Huskens J., Reinhoudt D.N.
    DOI

    Complexation of porphyrin-appended guests by calix[4]arene-appended cyclodextrins

    Journal of Inclusion Phenomena, vol. 41, (no. 1-4), pp. 163-172
  • Fiammengo R., Licini G., Nicotra A., Modena G., Pasquato L., Scrimin P., Broxterman Q.B., Kaptein B.
    DOI

    Duality of mechanism in the tetramethylfluoroformamidinium hexafiuorophosphate-mediated synthesis of N-benzyloxycarbonylamino acid fluorides

    Journal of Organic Chemistry, vol. 66, (no. 17), pp. 5905-5910
  • Fiammengo R., Crego-Calama M., Reinhoudt D.N.
    DOI

    Synthetic self-assembled models with biomimetic functions

    Current Opinion in Chemical Biology, vol. 5, (no. 6), pp. 660-673
  • 2000
  • Fiammengo R., Timmerman P., De Jong F., Reinhoudt D.N.
    DOI

    Highly stable cage-like complexes by self-assembly of tetracationic Zn(II) porphyrinates and tetrasulfonatocalix[4]arenes in polar solvents

    Chemical Communications, (no. 23), pp. 2313-2314
  • Peggion C., Flammengo R., Mossel E., Broxterman Q. B., Kaptein B., Kamphuis J., Formaggio F., Crisma M., Toniolo C.
    DOI

    Mag: A Cα-methylated, side-chain unsaturated α-amino acid. Introduction into model peptides and conformational preference

    Tetrahedron, vol. 56, (no. 22), pp. 3589-3601

Awards

2017  Italian National Scientific Habilitation (ASN) as full professor in Organic Chemistry (from 06/12/2017 until 06/12/2023)

2014  Italian National Scientific Habilitation (ASN) as associate professor in Organic Chemistry (from 14/11/2014 until 14/11/2020)