openfiberphotometry

The three-dimensional signal collection field for
fiber photometry in brain tissue

Marco Pisanello1,†,*, Filippo Pisano1,†, Minsuk Hyun2,†, Emanuela Maglie1,3, Antonio Balena1,3, Massimo De Vittorio1,3, Bernardo L. Sabatini2,*, and Ferruccio Pisanello1,*

1 Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies (CBN), Italy.
2 Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, U.S.A.
3 Dipartimento di Ingegneria dell’Innovazione, Università del Salento, Italy.

These authors have contributed equally to this work.

* Correspondence to: Marco Pisanello (This email address is being protected from spambots. You need JavaScript enabled to view it.), Bernardo L. Sabatini (This email address is being protected from spambots. You need JavaScript enabled to view it.), Ferruccio Pisanello (This email address is being protected from spambots. You need JavaScript enabled to view it.).

Frontiers in Neuroscience, 13:82. doi: 10.3389/fnins.2019.00082.

If you reuse data, codes, or models from this webpage, please cite: M. Pisanello, F. Pisano, M. Hyun, E. Maglie, A. Balena, M. De Vittorio, B.L. Sabatini, and F. Pisanello. The three-dimensional signal collection field for fiber photometry in brain tissue. Front. Neurosci. 13:82 (2019). doi: 10.3389/fnins.2019.00082.

RAW DATA

Click here to download collection fields in quasi transparent fluorescent medium. Within the zip archive you’ll find raw data (microscope and fiber acquired tiff stacks and results from ray-tracing simulations) and Matlab code for their processing, organized in different folders by fiber NA (0.22, 0.39, and 0.50) and sample number (A, B, C, and D).

Click here to download collection fields in fluorescently stained brain slice. Within the zip archive you’ll find raw data (microscope and fiber acquired tiff images) and Matlab code for their processing, organized in different folders by fiber NA (0.39 and 0.50) and sample number (A, B, and C).

Click here to download photometry efficiency in fluorescently stained brain slice. Within the zip archive you’ll find raw data (non de-scanned microscope and fiber acquired tiff images and through de-scanned pinhole images) and Matlab code for their processing, organized in different folders by fiber NA (0.39 and 0.50).

ZEMAX MODEL

Click here to download Zemax models for determining light collection fields in homogeneous and scattering medium. Within the zip archive you’ll find the Zemax lens models (zmx files) and macros (zpl files), and a guidelines document.

MATLAB FUNCTIONS

Click here to download the definition of Matlab functions required from the processing scripts.